Darja Semenistaja defeated Linda Klimovicova 6–3, 7–6 in the second qualifying round of the US Open on hard court on 22 August 2025. Semenistaja controlled the opening set with relative ease, breaking through in the first set before facing stiffer resistance in the second. Klimovicova mounted a competitive challenge in the decider, forcing a tie-break, but Semenistaja held her nerve in the decisive moments to secure passage to the final qualifying round. The victory keeps Semenistaja's hopes of reaching the main draw alive.